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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
15/01/2020 |
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Actualizado : |
16/01/2020 |
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Tipo de producción científica : |
Abstracts/Resúmenes |
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Autor : |
FLEITAS, A.L.; GALLINO, J.; SEÑORALE, M.; BONNECARRERE, V.; VIDAL, S. |
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Afiliación : |
A. L. FLEITAS, Laboratorio de Biología Molecular Vegetal, Facultad de Ciencias; JUAN GALLINO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; M. SEÑORALE, Sección Bioquímica, Facultad de Ciencias; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; S. VIDAL, Laboratorio de Biología Molecular Vegetal, Facultad de Ciencias. |
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Título : |
Optimización de técnicas de edición genómica libres de DNA en soja. [Resumen] |
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Complemento del título : |
Biotecnología Vegetal. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
In: REDBIO; INIA (Instituto Nacional de Investigación Agropecuaria); REDBIO Argentina. X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria y XI Simposio Redbio Argentina. Libro de Resúmenes. Montevideo 12 - 15 Noviembre 2019. Montevideo (UY): INIA, 2019. p. 19. |
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Serie : |
(INIA Serie Técnica; 253) |
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ISBN : |
e-ISBN 978-9974-38-437-8 |
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ISSN : |
1688-9266 |
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DOI : |
http://doi.org/10.35676/INIA/ST.253 |
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Idioma : |
Español |
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Contenido : |
Las metodologías de edición genómica, especialmente aquellas basadas en el sistema CRISPR/Cas9 se han establecido en los últimos años como herramientas poderosas al servicio del mejoramiento vegetal.
El trabajo pretende abordar dos interrogantes: por un lado, qué forma de Cas9 es más eficiente utilizar, la proteína o su mRNA; por otro lado, qué tejido y metodología de entrega es más efectiva. |
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Palabras claves : |
EDICIÓN GENÓMICA; SISTEMA CRISPR/Cas9. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13999/1/st-253-p19.pdf
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Marc : |
LEADER 01316nam a2200217 a 4500
001 1060605
005 2020-01-16
008 2019 bl uuuu u01u1 u #d
022 $a1688-9266
024 7 $ahttp://doi.org/10.35676/INIA/ST.253$2DOI
100 1 $aFLEITAS, A.L.
245 $aOptimización de técnicas de edición genómica libres de DNA en soja. [Resumen]$h[electronic resource]
260 $aIn: REDBIO; INIA (Instituto Nacional de Investigación Agropecuaria); REDBIO Argentina. X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria y XI Simposio Redbio Argentina. Libro de Resúmenes. Montevideo 12 - 15 Noviembre 2019. Montevideo (UY): INIA, 2019. p. 19.$c2019
490 $a(INIA Serie Técnica; 253)
520 $aLas metodologías de edición genómica, especialmente aquellas basadas en el sistema CRISPR/Cas9 se han establecido en los últimos años como herramientas poderosas al servicio del mejoramiento vegetal. El trabajo pretende abordar dos interrogantes: por un lado, qué forma de Cas9 es más eficiente utilizar, la proteína o su mRNA; por otro lado, qué tejido y metodología de entrega es más efectiva.
653 $aEDICIÓN GENÓMICA
653 $aSISTEMA CRISPR/Cas9
700 1 $aGALLINO, J.
700 1 $aSEÑORALE, M.
700 1 $aBONNECARRERE, V.
700 1 $aVIDAL, S.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
25/04/2018 |
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Actualizado : |
25/04/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GALLINO, J.P.; RUIBAL, C.; CASARETTO, E.; FLEITAS, A.L.; BONNECARRERE, V.; BORSANI, O.; VIDAL, S. |
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Afiliación : |
JUAN P. GALLINO, Universidad de la República (UdelaR)/ Facultad de Ciencias; CECILIA RUIBAL, Universidad de la República (UdelaR)/ Facultad de Ciencias; ESTEBAN CASARETTO, Universidad de la República (UdelaR)/ Facultad de Agronomía; ANDREA L. FLEITAS, Universidad de la República (UdelaR)/ Facultad de Ciencias; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OMAR BORSANI, Universidad de la República (UdelaR)/ Facultad de Agronomía; SABINA VIDAL, Universidad de la República (UdelaR)/ Facultad de Ciencias. |
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Título : |
A dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 2018, v.9, Article number 262. (2 March 2018). OPEN ACCESS |
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DOI : |
10.3389/fpls.2018.00262 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018. |
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Contenido : |
ABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress.
© 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal. MenosABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (Gm... Presentar Todo |
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Palabras claves : |
ABIOTIC STRESS; ARABIDOPSIS; DROUGHT; EIFiso4G; SOYBEAN CROP; TRANSLATION INITIATION. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9385/1/Frontiers-in-Plant-Science.-2018.fpls-09-00262.pdf
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Marc : |
LEADER 03453naa a2200289 a 4500
001 1058480
005 2018-04-25
008 2018 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2018.00262$2DOI
100 1 $aGALLINO, J.P.
245 $aA dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis.$h[electronic resource]
260 $c2018
500 $aArticle history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018.
520 $aABSTRACT. Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress. © 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal.
653 $aABIOTIC STRESS
653 $aARABIDOPSIS
653 $aDROUGHT
653 $aEIFiso4G
653 $aSOYBEAN CROP
653 $aTRANSLATION INITIATION
700 1 $aRUIBAL, C.
700 1 $aCASARETTO, E.
700 1 $aFLEITAS, A.L.
700 1 $aBONNECARRERE, V.
700 1 $aBORSANI, O.
700 1 $aVIDAL, S.
773 $tFrontiers in Plant Science, 2018$gv.9, Article number 262. (2 March 2018). OPEN ACCESS
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